Get Cell Culture Techniques in Heart and Vessel Research PDF

By A. C. Nag (auth.), Prof. Dr. Dr. H. M. Piper (eds.)

ISBN-10: 3642752624

ISBN-13: 9783642752629

ISBN-10: 3642752640

ISBN-13: 9783642752643

In the cardiovascular sciences, an expanding call for for using glossy equipment of mobilephone biology has constructed. using particular cellphone tradition versions of many of the tissues concerned is key for many of those novel ways. This ebook meets the call for for acomprehensive and straightforward obtainable resource for mobile cul- ture equipment in cardiovascular examine because it used to be now not formerly on hand. the fundamental tools for cultures of cardiomyocytes (embryonic and adult), endothelial cells (micro- and macrovascular), delicate muscle cells and pericytes are defined intimately via a global choice of specialists. specified chapters talk about using progress components and attachment substrates, thoughts for co-cultures, cultures on permeable clear out membranes and microcarrier cultures. The methodological descriptions are sufficiently specific for an instantaneous program within the laboratory. All chapters additionally comprise a severe evaluate of different approaches.

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J Cell Bioi 31: 131 A 37. Kasten FH (1972) Rat myocardial cells in vitro: mitosis and differentiated properties. In Vitro 8:128-150 38. Kasten FH, Yip DK (1974) Reamination of cultured mammalian myocardial cells during multiple cycles of trypsinization freezing-thawing. In Vitro 9:246-252 39. Kessler-Icekson G (1987) Cardiomyocytes grown in serum-free medium. In: Pinson A (ed) The heart cell in culture. CRC, Boca Raton, pp 23-28 40. Kessler-Icekson G, Wasserman L, Yoles E, Aampson SR (1983) Characterization of cardiomyocytes cultured in serum-free medium.

Waelllng ,, ,, , Stirring for 15 - 20 min. , ) Stirring for 15 - 20 min. ICentrlfugallon I . . '--_'-'----' . . Stirring for 15 - 20 min. 1on ~ of combined ' - ~. m I .... -__ Idem -'I . . Idem I ......... 1 _Idem _----'I . . m--,I . . I I~forl celcOWII 1.... -----. ling Fig. 3. Schematic representation of culture preparation methods. [From 64] Supernatant decanted 3:: c :; n '< n i" ~ ":;. t 0- " II Neonatal Rat Heart Muscle Cells 29 Cells in each tube are suspended by repeated aspirations in a sterile pipette and all the fractions combined in a sterile 250 ml flask, passing them through a sterile mesh in order to exclude explants.

Biochim Biophys Acta 115:15-22 31. Harary I, Hoover F, Farley B (1974) The isolation and cultivation of rat heart cells. Enzymol 32:740-745 32. Harary I, Renaud J-F, Wallace G (1976) Ca ions regulate cyclic AMP and beating in cultured heart cells. Nature 261:60-61 33. Holtzer H (1979) Myogenesis. In: Schjeide QA, de Vellis I (eds) Cell differentiation. Van Nostrand Reinhold, New York, pp 476-503 34. Houba V (1967) The use of pronase for dispersing cells. Experientia 23: 572 35. Kaneko H, Goshima K (1982) Selective killing of fibroblast like cells in cultures of mouse heart cells by treatment with CA ionophore A 23187.

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Cell Culture Techniques in Heart and Vessel Research by A. C. Nag (auth.), Prof. Dr. Dr. H. M. Piper (eds.)


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