Download e-book for kindle: Cell Fusion: Overviews and Methods by Kurt Pfannkuche

By Kurt Pfannkuche

ISBN-10: 1493927027

ISBN-13: 9781493927029

ISBN-10: 1493927035

ISBN-13: 9781493927036

This quantity information protocols on detection and research of fusion occasions in quite a few mobilephone forms in vitro and in vivo. extra, protocols target to facilitate the longer term hugely proper strategy of engineering cells for particular reasons, similar to new release of transgenic embryos and improvement of hybrid cells, that allows you to take on particular projects in mobilephone biology and drugs. Written for the Methods in Molecular Biology sequence, chapters comprise introductions to their respective themes, lists of the required fabrics and reagents, step by step, without problems reproducible laboratory protocols, and pointers on troubleshooting and averting recognized pitfalls.

Practical and authoritative, Cell Fusion: Overviews and techniques, moment Edition serves as a great reference on mobile fusion and laboratory protocols.

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90. 91. in vivo. Proc Natl Acad Sci U S A 105: 15446–15451 O'Brien SP, Seipel K, Medley QG, Bronson R, Segal R, Streuli M (2000) Skeletal muscle deformity and neuronal disorder in Trio exchange factor-deficient mouse embryos. Proc Natl Acad Sci U S A 97:12074–12078 Kim S, Shilagardi K, Zhang S, Hong SN, Sens KL, Bo J, Gonzalez GA, Chen EH (2007) A critical function for the actin cytoskeleton in targeted exocytosis of prefusion vesicles during myoblast fusion. Dev Cell 12:571–586 Dalkilic I, Schienda J, Thompson TG, Kunkel LM (2006) Loss of FilaminC (FLNc) results in severe defects in myogenesis and myotube structure.

J Cell Sci 8:681 81. Yap TA, Gerlinger M, Futreal PA, Pusztai L, Swanton C (2012) Intratumor heterogeneity: seeing the wood for the trees. Sci Transl Med 4:127ps10 82. Greaves M, Maley CC (2012) Clonal evolution in cancer. Nature 481:306 83. Gerlinger M et al (2012) Intratumor heterogeneity and branched evolution revealed by multiregion sequencing. N Engl J Med 366:883 84. Mullighan CG et al (2008) Genomic analysis of the clonal origins of relapsed acute lymphoblastic leukemia. Science 322:1377 85.

If two or more cells fuse together, they can either build heterokaryons—containing both nuclei separately— or the nuclei fuse generating synkaryons [48] (Fig. 1). In human tumors both can occur [49–51]. One prominent trigger for cell fusion might be high risk oncogenic human Papillomavirus (HPV) infection, the main cause of cervical cancer [52]. Binucleated cells are strongly observed in almost any HPV-positive low-grade squamous intraepithelial lesions before leading to accumulation of chromosomal aberrations in polyploid cells and co-overexpression of p53 and bcl-2 to high-grade squamous intraepithelial lesions [49, 53, 54].

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Cell Fusion: Overviews and Methods by Kurt Pfannkuche

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