By Kurt Pfannkuche
This quantity information protocols on detection and research of fusion occasions in quite a few mobilephone forms in vitro and in vivo. extra, protocols target to facilitate the longer term hugely proper strategy of engineering cells for particular reasons, similar to new release of transgenic embryos and improvement of hybrid cells, that allows you to take on particular projects in mobilephone biology and drugs. Written for the Methods in Molecular Biology sequence, chapters comprise introductions to their respective themes, lists of the required fabrics and reagents, step by step, without problems reproducible laboratory protocols, and pointers on troubleshooting and averting recognized pitfalls.
Practical and authoritative, Cell Fusion: Overviews and techniques, moment Edition serves as a great reference on mobile fusion and laboratory protocols.
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Extra resources for Cell Fusion: Overviews and Methods
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If two or more cells fuse together, they can either build heterokaryons—containing both nuclei separately— or the nuclei fuse generating synkaryons  (Fig. 1). In human tumors both can occur [49–51]. One prominent trigger for cell fusion might be high risk oncogenic human Papillomavirus (HPV) infection, the main cause of cervical cancer . Binucleated cells are strongly observed in almost any HPV-positive low-grade squamous intraepithelial lesions before leading to accumulation of chromosomal aberrations in polyploid cells and co-overexpression of p53 and bcl-2 to high-grade squamous intraepithelial lesions [49, 53, 54].
Cell Fusion: Overviews and Methods by Kurt Pfannkuche