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By Susan Hawkes (Eds.)

ISBN-10: 0123333202

ISBN-13: 9780123333209

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2, D. , 339 Cell Hughes, R. , Physiol. Biol. , Biol. Internat. (1978). , Molec. Schlesinger, Cell. Biochem. , 36, (1981). Hayashi, 11292 43. J · Lai, R. Schroeder, Marquette, 147 42. , Credo, (1979). Pena, Rep. Κ. , 427 O . , Molnar, G. 87, 360, Yamada, 492 40. , Water, R. Jilek, Chem. 39. P. , and Κ. , J . Biol. Chem. 256, (1981). Atherton, Β. Hynes, R. O . , Cell 25, 133 (1981). STRUCTURE AND BIOSYNTHESIS OF CARTILAGE COLLAGENS 1 M i c h a e l D. S u s s m a n Roy C . O g l e Sandra L. Hubbard Gary B a l i a n Departments o f O r t h o p e d i c s , P e d i a t r i c s and B i o c h e m i s t r y U n i v e r s i t y of V i r g i n i a School of Medicine C h a r l o t t e s v i l l e , V i r g i n i a 22908 I.

5M NaCl e x t r a c t ( F i g u r e 1 ) . Type IV c o l l a g e n c o u l d o n l y be e x t r a c t e d i n q u a n t i t y from l a t h y r i t i c t i s s u e i n 2M g u a n i d i n e w i t h a r e d u c i n g a g e n t t o c l e a v e d i s u l f i d e bonds ( T a b l e I I ) . This r e s u l t i n d i c a t e s t h a t b o t h d i s u l f i d e bonds and l y s i n e d e r i v e d c r o s s l i n k s o c c u r b e t w e e n t y p e IV c o l l a g e n m o l e c u l e s and s t a b i l i z e t h e i r s t r u c t u r e .

S. Α . , M a d r i , J . Α . , Mol. Biol. , J . Μ. , V. , (1980). Τ . , Ν · , and Saraste, Eur. J. G i l b e r t , Soc. , Edelhoch, Alexander, B e j a n i a n , C a r r e l l , V a r t i o , S. and McDonagh, J. J . , Cell (1981). Vuento, I . , 31. , H. 91, Amer. 30. V. Biol. , ). , 254, 77, G. 157 J . Α . , Biol. Colonna, 1501 von and 105, B o n s d o r f f , 33 Scheraga, C - (1980). Η. Α . , J . (1955). , Biochem. , (1979). Yamada, Chem. and Κ. , (1978). J . KENNETH Μ. YAMADA 34 32. Cantor, try, 33.

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