HPLC of Peptides and Proteins, Methods and Protocols - download pdf or read online

By Marie-Isabel Aguilar

ISBN-10: 0896039773

ISBN-13: 9780896039773

Hands-on specialists from academia and comprehensively describe find out how to effectively practice the entire severe HPLC concepts wanted for the research of peptides and proteins.

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Extra resources for HPLC of Peptides and Proteins, Methods and Protocols

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4. Inject the protein sample, start the fraction collector (1 min fractions), and continue washing the column until the UV trace returns to baseline (see Fig. 3). 5. Start the gradient (see Note 8). At completion of the gradient, continue to wash the column with five column volumes of the elution buffer (5 mL), then apply five CH03,23-44,22pgs 10/30/03 6:58 PM Page 39 Ion-Exchange Chromatography 39 column volumes of stripping buffer (1 M NaCl) to elute any strongly bound material. 6. Reequilibrate the column with 5–10 column volumes of starting buffer.

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HPLC of Peptides and Proteins, Methods and Protocols by Marie-Isabel Aguilar


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