Read e-book online Parlons Biélorussien : Langue et culture PDF

By Virginie Symaniec, Alexandra Goujon

ISBN-10: 2738458440

ISBN-13: 9782738458445

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3. 4. 1. Preparation of Calibration Curve Pipet 25,50,100,150,200,300, and 400 PL aliquots of standard phosphate solution into duplicate sets of test tubes. Make up all volumes to 500 PL and mix well. 5% ammonium molybdate solution to each tube. 8 mL of distilled water and mix well. Add 200 PL of reducing agent working solution at timed intervals to each tube and mix well. Incubate the tubes at room temperature for 10 min, and read the absorbance at 660 nm. Construct a calibration curve of phosphate concentrations @mol P,> against absorbance readings.

Fructose Measurement This method uses 70% H,SO, prepared as described above. 1. Preparation of Calibration Curve It is advisable to construct the calibration curve prior to the start of medium analysis, since it is easier to detect whether anything is wrong with the assay at this stage. 22 Stepan-Sarkissian and Grey 1. Pipet 20,40,60,80, and 100 PL aliquots of standard fructose solution into duplicate sets of tubes, and make up the volume in each tube to 100 PL with distilled water. 2. At timed intervals (seesection on Sucrose Measurement above), add 3 mL of anthrone reagent, and leave at room temperature for 15 min.

1. Preparation of 70% H2S04 NOTE: The preparation of 70% H2S04 from concentrated acid is an extremely exothermic reaction, and certain precautions need to be observed. 1. Wear safety glasses and gloves, and keep a container of Na,CO, at hand to be used in the event of acid spillage. 2. Embed a 2-L conical flask containing a magnetic flea in ice in a plastic bowl. Place the bowl on a magnetic stirrer in a fume cupboard. 3. Pour 300 mL distilled water into the flask, and switch on the stirrer. 21 Growth Determination 4.

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Parlons Biélorussien : Langue et culture by Virginie Symaniec, Alexandra Goujon


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