Get RNA Isolation and Characterization Protocols (Methods in PDF

By Ralph Rapley, David L. Manning

ISBN-10: 0896033937

ISBN-13: 9780896033931

Univ. of Hertfordshire, Hatfield, united kingdom. textual content providing organic investigators a set of up-todate tools for the isolation of RNA from numerous resources, together with: bacterial, plant, and mammalian cells. transformed define structure. Plastic-comb binding, hardcover to be had.

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Additional info for RNA Isolation and Characterization Protocols (Methods in Molecular Biology)

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5. 6. 7. 8. 9. Mortar and pestle. Liquid nitrogen. 10-mL Nalgene Oak Ridge polypropylene Preparative centrifuge and microfuge. 5~mL micromge tubes. tube, autoclaved. least 3 h. tubes, autoclaved. tubes. 3. Methods 1. 4 g of tissue in a mortar and pestle to a tine powder m liquid nitrogen. Do not allow the tissue to thaw. 2. 5. mL of extractron buffer and grind thoroughly while the tissue thaws Transfer the homogenate to a 10-mL Oak Ridge tube. Rinse the mortar and pestle with 1 mL of extraction buffer and then transfer to the tube.

Furthermore, blood constituents or their derivatives may inhibit PCR reactions (1) RNA extraction from blood is therefore usually more successful if the nucleated white cells are first isolated from the red cells. As with extraction from other tissues,it is important to minimize degradation by following the appropriate recommendations for handling RNA, as detailed in the methodology below. A variety of methods are employed for the extraction of RNA (2), which usually comprise cell lysis, partitioning of RNA into a solvent fraction, and recovery of RNA from the solvent by precipitation.

Ohgodeoxthymidylate-cellulose (oligo[dT]): Ohgo (dT) cellulose is available commercially.

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RNA Isolation and Characterization Protocols (Methods in Molecular Biology) by Ralph Rapley, David L. Manning


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